Real-time reverse transcription loop-mediated isothermal amplification for rapid detection of SARS-CoV-2

Rapid Detection of West Nile Virus for Loop-Mediated Isothermal Amplification Real-Time Reverse Transcription

Real-time reverse transcription loop-mediated isothermal amplification for rapid detection of West Nile virus.

A one-step, single tube, real-time accelerated reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed for detecting the envelope gene of West Nile (WN) virus. The RT-LAMP assay is a novel method of gene amplification that amplifies nucleic acid with high specificity, efficiency, and rapidity under isothermal conditions with a set of six specially designed pri...

Real - Time Detection of HIV - 2 by Reverse Transcription , Loop - Mediated 1 Isothermal Amplification

Real-Time Detection of HIV-2 by Reverse Transcription, Loop-Mediated 1 Isothermal Amplification 2 3 Kelly A. Curtis*, Philip Niedzwiedz, Ae S. Youngpairoj, Donna L. Rudolph, and S. Michele 4 Owen 5 6 Laboratory Branch, Division of HIV/ AIDS Prevention, National Center for HIV/AIDS, 7 Hepatitis, STD, and TB Prevention, Centers for Disease Control and Prevention, Atlanta, GA, 8 30333 9 10 The fin...

Real-Time Detection of HIV-2 by Reverse Transcription-Loop-Mediated Isothermal Amplification.

Currently, there are no FDA-approved nucleic acid amplification tests (NAATs) for the detection or confirmation of HIV-2 infection. Here, we describe the development of a real-time assay for the detection of HIV-2 DNA and RNA using reverse transcription-loop-mediated isothermal amplification (RT-LAMP) and the ESEQuant tube scanner, a portable isothermal amplification/detection device.

Rapid detection of infectious bursal disease by loop-mediated isothermal amplification for field analysis

Infectious bursal disease (IBD) is an immunosuppressive, acute and highly contagious illness of growing-poultry stock infected with infectious bursal disease virus (IBDV). It is common in Pakistan, causing potential economic losses throughout the year. The objective of the study is to propose a rapid, sensitive and specific diagnostic tool, and compare it with existing commonly used reverse tra...